MS-DOS Player for Win32-x64 (2014/11/30) is released. MS-DOS Player is MS-DOS emulator running on Win32-x64 command prompt. 16bit MS-DOS compatible commands can be exexuted on Win32-x64 envrionment.

MS-DOS Player for Win32-x64 (2014/11/30) Changelog (Google Translate):

- support keyboard controller to control a20 line - support int21h ax=5800h/5801h/5802h/5803h

Download: MS-DOS Player tested on Windows 7 (x64)(2014/11/30)
Source: Here