MS-DOS Player for Win32-x64 (2012/08/23) is released. MS-DOS Player is MS-DOS emulator running on Win32-x64 command prompt. 16bit MS-DOS compatible commands can be exexuted on Win32-x64 envrionment.

MS-DOS Player for Win32-x64 (2012/08/23) Changelog (Google Translate):

I replaced the i386 0.146u3 based MAME, the CPU core. I also support (the internal address of the acquisition of the work area DOS) int 21h ah = 52h. Thus, DDEB was i386 is needed to work. Support for DOS-EXTENDER is undecided for now.

Download: MS-DOS Player for Win32-x64 (2012/08/23)
Source: Here