MS-DOS Player for Win32-x64 (2015/04/24) is released. MS-DOS Player is MS-DOS emulator running on Win32-x64 command prompt. 16bit MS-DOS compatible commands can be exexuted on Win32-x64 envrionment.

MS-DOS Player for Win32-x64 (2015/04/24) Changelog (Google Translate):

- the i386 core, I have to apply the changes of up to MAME 0.160. - Since the correspondence, such as SSE instruction center, meaning too much in MS-DOS Player is unlikely.

Download: MS-DOS Player tested on Windows 7 (x64)(2015/04/24)
Source: Here