MS-DOS Player for Win32-x64 (2015/10/20) is released. MS-DOS Player is MS-DOS emulator running on Win32-x64 command prompt. 16bit MS-DOS compatible commands can be exexuted on Win32-x64 envrionment.

MS-DOS Player for Win32-x64 (2015/06/25) Changelog (Google Translate):

- support int 21h, ah=14h/15h/17h/23h/24h/27h/28h (not tested) - support int 10h, ax=1118h - add NEC V30 instructions

Download: MS-DOS Player tested on Windows 7 (x64)(2015/10/20)
Source: Here